Stem Cell Collection Methods

After tests confirm that you are eligible to donate, your stem cells will be collected in case a patient needs them.  There are two methods to collect stem cells:

1.  PBSC Collection

2.   Collection from Bone Marrow

The Peripheral Blood Stem Cell collection (PBSC) is more commonly used.


A.Peripheral Blood Stem Cell (PBSC) collection method: Apheresis

Before a PBSC collection procedure, the donor may receive a prescribed dose of drugs called growth factors. A common growth factor is Granulocyte Colony-Stimulating Factor (G-CSF). These drugs will generally cause the release of a large number of peripheral blood stem cells into the blood stream. You will receive the G-CSF drug for a number of days through injection. When the physician determines the white blood cell count has increased to a sufficient level, a series of PBSC collections will begin. On the collection day, needles or catheters will be inserted in both of your arms. The sterile tubing sets or needles are used only once and then discarded. Your blood will enter an Apheresis machine where stem cells will be separated from your blood in a quite sterile condition and then comes back to your body through your other

arm. It usually takes 3-4 hours. You will lie on a bed during the collection procedure.  It is to some extent like blood donation.  donors may feel somewhat dizzy, cold or nauseous during the procedure. Afterward, they may feel tired.

B.Collection from Bone Marrow

Surgically extracting the bone marrow for stem cell collection takes place in an operating room, where the donor will determine whether s/he will be under general anesthesia or just analgesia. During the surgery, the donor's bone marrow is harvested from several puncture sites in the hipbones using a special needle


Most donors will be discharged the same day, however some donors may stay at hospital overnight. The day after stem cell collection, you may be visited by a doctor.


You will not directly take benefits from your donation, but your stem cells will give a new life to the patient who receives them.


Stem cell collection will not have any risks for the donor. The most serious risk associated with donating bone marrow involves the use of anesthesia during the procedure. Unlike bone marrow donation, PBSC donation does not require anesthesia. Within a few days, the donor’s body replaces the donated cells. The donated marrow (in case of bone marrow harvest) will be replaced within a few weeks

Contact us for more information

Address:  Iranian Stem Cell Donor Program(ISCDP), Shariati Hospital, Kargar-e Shomali Ave., Tehran, Iran

Telephone: +98-21-88353028-88357017-88352830-88353036